Antigen

Antigen Class

TFs and others

Antigen

CDC73

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

lymphoblast from bone marrow

cell line

K562

tissue

lymphoblast from bone marrow

chip antibody

Parafibromin (rabbit, bethyl laboratories, A300-171A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

crosslink 5 min), harvest, 10% of NIH 3T3 spike in, lysis of cells, fragment with covaris libraries were prepared for sequencing using standard NEB protocols

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

38199613

Reads aligned (%)

90.6

Duplicates removed (%)

8.5

Number of peaks

3708 (qval < 1E-05)

hg19

Number of total reads

38199613

Reads aligned (%)

90.1

Duplicates removed (%)

9.9

Number of peaks

3536 (qval < 1E-05)